Ab initio phasing and chain tracing - ARCIMBOLDO

ARCIMBOLDO originally conceived for ab initio phasing of macromolecular structures.

There are two main barriers for ab initio phasing: structure size and resolution of the data. ARCIMBOLDO constitutes a general method for 2 Å data, based on a combination of small α-helices with Phaser ( to locate small model fragments) and SHELXE (density modification with and autotracing).

Please, choose the ARCIMBOLDO program to run:

ARCIMBOLDO_LITE performs ab initio phasing using polyalanine helices or other single search fragments. (http://chango.ibmb.csic.es/)

ARCIMBOLDO BORGES performs ab initio phasing with nonspecific libraries of small folds. (http://chango.ibmb.csic.es/borges)

ARCIMBOLDO_SHREDDER uses fragments derived from a distant homolog template. (http://chango.ibmb.csic.es/shredder)

Helical structures are preferable cases for phasing with ARCIMBOLDO

The program documentation for ARCIMBOLDO_LITE , ARCIMBOLDO BORGES and ARCIMBOLDO_SHREDDER